A Good Abcam Antibody for VPS53

August 14, 2019

Sir William Dunn School of Pathology
Oxford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti VPS53 antibody

Catalog Number:

ab251759

Clone Number:

Polyclonal

This antibody gave a clean band around the expected molecular weight; could stain the Golgi area localisation of this protein, but the staining was very weak and fuzzy.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse MEF

Primary Incubation

1:1000 in 1% milk in PBST at 4 degree overnight

Blocking Agent

1% milk in PBST at room temperature for 1 hour

Secondary Incubation

1:5000 in 1% milk in PBST at room temperature for 1 hour

Tertiary Incubation

N/A

Detection

ECL

Results Summary

This antibody gave a clean band around the expected molecular weight; could stain the Golgi area localisation of this protein, but the staining was very weak and fuzzy.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean band

The Bad

Very weak Golgi staining

The Bottom Line

Could be very good for WB, but may not good for IF staining.

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