A Poor Antibody for Smoothened

August 21, 2019

BlueskyReddit
Sir William Dunn School of Pathology
Oxford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti Smoothened antibody

Catalog Number:

ab236465

Clone Number:

Polyclonal

This antibody gave many nonspecific bands around the expected molecular weight. What is worse, none of these bands responded to knockdown of Smo.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human RPE1 cell lysates

Primary Incubation

1:1000 diluted in 1% milk in PBST, 4 degree overnight

Blocking Agent

1% milk in PBST at room temperature for 1 hour

Secondary Incubation

1:5000 diluted in 1% milk in PBST at room temperature for 1 hour

Tertiary Incubation

N/A

Detection

ECL

Results Summary

No specific bands were observed.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

None

The Bad

Not specific

The Bottom Line

A bad antibody

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