Good SOX2 Antibody for Glial Cells from R&D

Neuroscience
Wisconsin University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

SOX2 Antibody [Unconjugated]

Catalog Number:

AF2018

Clone Number:

Polyclonal

The aim of our research is to study a role played by glial cells in neurodegenerative diseases. The specific goal of this experiment was to quantify glial cells in the course of disease progression. This antibody demonstrated excellent staining for glial cells in the optic nerve.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Feline optic nerve tissues

Primary Incubation

Diluted at 1:500, overnight at 4°C

Blocking Agent

10% normal donkey serum + 0.5% triton X + 0.1M PBS

Secondary Incubation

Alexa 647 conjugated anti-goat IgG 1:500, 1 hr at RT

Tertiary Incubation

N/A

Detection

Fluorescence microscopy

Results Summary

Robust signals from immunolabeling with this antibody showing immunoreactivity in glial cells in feline optic nerve tissues. SOX2-magenta, DAPI-blue.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Robust staining with no un-specific binding

The Bad

None

The Bottom Line

Highly recommend as a glial marker in central nerve system.

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