Abcam Got1 Antibody Works Well

Pharmacology and Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti Aspartate Aminotransferase Antibody

Catalog Number:

Ab170950

Clone Number:

EPR12145

This is a rabbit monoclonal antibody. It worked for human and mouse for Western blot on our hands with low background and strong signal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse CD8 T cell

Primary Incubation

1:2000, OVN, 4 degree

Blocking Agent

5% milk, 1hr

Secondary Incubation

1:5000, RT, 1hr

Tertiary Incubation

None

Detection

ECL

Results Summary

Mouse CD8 T cells were transduced with retroviral EV, or shRNA against mouse GOT1 (shown as RV1, RV3 in the picture). GFP+ cells (indicating cells successfully transduced) were FACS sorted for western blot.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High signal-to-noise ratio

The Bad

Not ideal for flow cytometry

The Bottom Line

I recommend this product for western blot for human and mouse cells or tissue.

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