Review of Anti-human NKp44 (CD336) Biotin from BioLegend

July 30, 2019

BlueskyReddit
Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Anti-human NKp44 (CD336) Biotin

Catalog Number:

325106

Clone Number:

P44-8

Activating NK receptor NKp44 (CD336) is a natural cytotoxicity triggering receptor member of the natural cytotoxicity receptor family. NKp44 is expressed on IL-2 activated NK cells, a subset of γ/δ T cells, and a subset of human ILC3s. NKp44 enhances NK cell mediated cytolysis of virus infected cells and tumor cells, and activates expressing ILCs to secrete cytokines.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human ILCs

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

10min, 10°c with Streptavidin

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Good resolution in both cultured cells and fresh tissues.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific, good resolution

The Bad

Not the brightest depending on which streptavidin is utilized

The Bottom Line

Good product, easy to optimize, and good resolution.

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