Thermo Fisher Scientific
Lipofectamine 3000 Transfection Reagent
L3000008
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Transfection using RNA or DNA is a common cell culture tool. We used Lipofectamine 3000 to silence TNFa in HL-1 cardiomyocytes. Lipofectamine 3000 has relatively less cell toxicity and very high transfection efficiency.
Cell culture
Cardiomyocytes
6-24 h transfection showed no toxicity.
Lipofectamine 3000 transfected cells showed ~70% reduction in TNFa expression levels.
N/A
Worked as expected
Lipofectamine 3000 can be used as a tool to overexpress or knock down genes in various cell type.