Beta 3 Tubulin Antibody from CST Is Very Good

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

β3-Tubulin (D71G9) XP® Rabbit mAb

Catalog Number:

5568

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. β3-Tubulin is a classic neuron marker. I want to stain the retina ganglion neuron in cryostat sections.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse retina cryostat sections

Primary Incubation

1:100, overnight at 4°C

Blocking Agent

PBST (0.3%) containing 10% goat serum

Secondary Incubation

1:200, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software

Results Summary

The signal is sensitive and specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody works well.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

Share your experience with other scientists. Write a Review! »

Join the discussion