Karyopherin Alpha2 Antibody from Santa Cruz Is Good

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Karyopherin α2 Antibody (B-9)

Catalog Number:

sc-55538

Clone Number:

B-9

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of Kpna2 in mouse oligodendrocyte cell culture.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse oligodendrocyte cell culture

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:1000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

Membranes were incubated with ECL (Thermo Scientific, Somerset, NJ) for 10 min and then revealed

Results Summary

The band is sensitive and specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody works well.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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