Importin Alpha5 Antibody from Proteintech Is Satisfactory

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

importin α5 Antibody

Catalog Number:

18137-1-AP

Clone Number:

Polyclonal

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of importin α5 in oligodendrocyte cell culture.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse oligodendrocyte cell culture

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:1000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

Membrances were incubated with ECL (Thermo Scientific, Somerset, NJ) for 10 min and then revealed

Results Summary

The band looks ok but the background is a little bit dirty.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody is ok.

The Bad

The background is a little bit dirty.

The Bottom Line

I still recommend this antibody.

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