GAPDH Antibody from Sigma Is Very Good

July 31, 2019

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

MilliporeSigma

Product Name:

Anti-GAPDH antibody, Mouse monoclonal

Catalog Number:

G8795

Clone Number:

GAPDH-71.1

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of Gapdh in mouse dorsal root ganglion.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:10000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

This antibody is very sensitive and specific. It can be used at rather low concentration.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is specific and sensitive.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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