Ezh2 Antibody from CST Is Satisfactory

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Ezh2 (D2C9) XP® Rabbit mAb #5246

Catalog Number:

5246

Browse Similar Products: Ezh2 Western Blot Antibodies

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of Ezh2 in mouse dorsal root ganglion.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

There are two bands, the lower one is the target band of Ezh2.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is ok

The Bad

There is an unspecific band

The Bottom Line

This antibody works ok.

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