Phospho-Akt Antibody from CST Is Very Good

July 17, 2019

BlueskyReddit
Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rabbit anti-pAkt Ser473

Catalog Number:

4060

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to detect the expression of pAkt Ser473 in mouse dorsal root ganglion.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse dorsal root ganglion

Primary Incubation

1:2000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 5% blotting-grade blocker

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The band is specific.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is specific and strong.

The Bad

N/A

The Bottom Line

I definitely recommend this antibody.

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