IL-4 Detection in LPA-Treated Microglia Cells (Novus)

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Novus Biologicals

Product Name:

IL-4 Antibody

Catalog Number:

AF-404-SP

Clone Number:

N/A

Primary murine microglia were treated with LPA 1µM and the levels of IL-4 expression were analyzed using immunoblotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Primary murine microglia

Primary Incubation

1/500, O/N at 4°C

Blocking Agent

5% milk

Secondary Incubation

Rabbit anti-goat, 1/5000, 2h at RT

Tertiary Incubation

N/A

Detection

ECL+, ChemiDoc™ Imaging System

Results Summary

Treatment with LPA decreased the expression of IL-4 in primary murine microglia.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, reproducible results

The Bad

None

The Bottom Line

A good Ab for the detection of IL-4 in microglia cells.

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