Changes in CD206 Expression in Microglia Cells

July 18, 2019

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PE anti-mouse CD206 (MMR) Antibody

Catalog Number:

141706

Clone Number:

C068C2

BV2 microglia cells were treated for different time points with 1µM LPA. Differences in the expression of the mannose receptor (MR), CD206 were observed using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

BV2 microglia cells

Primary Incubation

1/100, 40min at 4°C

Blocking Agent

5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Treatment with LPA did not affect the expression levels of CD206 after 12 and 24 hours. A significant decrease in the CD206 levels was observed after 48 hours treatment.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, reproducability of the results

The Bad

None

The Bottom Line

A good antibody for the detection of CD206.

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