Detection of CD40 Expression in Microglia Cells

July 11, 2019

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

PE anti-mouse CD40 Antibody

Catalog Number:

124610

Clone Number:

3/23

BV2 microglia cells were treated with LPA (1µM) for different time points and the expression of CD40 surface marker was analyzed using flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

BV2 microglia cells

Primary Incubation

1/50, 40min at 4°C

Blocking Agent

5% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

Treatment with LPA induced an increase in the expression of CD40.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, reproducible results

The Bad

None

The Bottom Line

A reliable conjugated antibody for the detection of CD40.

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