Abcam
DCFDA / H2DCFDA Cellular ROS Assay Kit
ab113851
Info:Supplier Page View Product Specs View Product
Primary murine microglia cells were incubated for 45min with 20µM DCFDA, then treated for the indicated time points with 1µM LPA in the presence or absence of an LPAR5 inhibitor. The fluorescence intensity was measured using a Wallac Victor 1420 Multilabel Counter.
Assay kit
Primary murine microglia
The concentration of DCFDA that will be used is based on the type of cells and should be optimized.
LPA treated microglia cells had increased levels of ROS. Inhibition of LPAR5 using a specific inhibitor (TCLPA5 4) significantly decreased this LPA induced ROS production.
N/A
Easy to use
There is quite a variation between triplicates.
Generally a good kit for the detection of cellular ROS.