BioLegend Antibody Works Well for F4/80

Immunology
Duke
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

F4/80 BV 650

Catalog Number:

123149

Clone Number:

BMA

Our main research aims are as follows: (1) to understand the role of necroptosis signal adaptors in inflammatory diseases and (2) to elucidate the role of cell death in anti-viral immune responses. This antibody worked well but did have some compensation issues wit BV 421.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine PBMC's

Primary Incubation

1:75

Blocking Agent

Fc

Secondary Incubation

None

Tertiary Incubation

None

Detection

BS LSR2

Results Summary

This antibody worked well for detection of BV650 F4/80 macrophages and co stained with Cd11b.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works well

The Bad

Compensation issues

The Bottom Line

Overall this worked well and stained for the intended protein/population.

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