CD25 Antibody Only Works Pre-Fixation

August 28, 2019

Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BioLegend

Product Name:

PE Anti CD25

Catalog Number:

302606

Clone Number:

BC96

Our lab is interested in new treatments for autoimmune diseases and inflammation. For my research, I have developed novel inhibitors of kinases in the nfkb pathway. This antibody only works well pre fixation. PFA fixation prevented good staining.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1:100

Blocking Agent

Fc

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR2

Results Summary

Works pre-fixation only.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Works pre-fixation

The Bad

Not for post-fixed samples

The Bottom Line

Optimize with cell processing.

Share your experience with other scientists. Write a Review! »

Join the discussion