Lonza
Human Stem Cell Nucleofector Kit
VPH-5012
Our aim is to successfully transfect cancer stem cells with CRISPR plasmids to generate knock-ins of specific proteins. We transfected cancer stem cells with CRISPR plasmids and template using human stem cell nucleofector reagents. 48 h after transfection cell were sorted based on GFP expression and successfully generated single clones of knock-ins after screening.
Stem cell transfection
Cancer stem cells
We used nucleofector 2b device from Lonza for nucleofection
Successfully generated multiple clones of knock-ins with one round of transfection
N/A
Least toxic to cells
Cancer stem cells are hard to transfect using lipofectamine and toxic to cells. This kit increased the efficiency of transfection and non-toxic to stem cells.