Sigma-Aldrich
Mouse anti-β-actin
A1978
AC-15
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Browse Similar Products: Beta-Actin Western Blot Antibodies
My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this western blot experiment, I want to use the expression of β-actin as an internal reference.
Western Blot
Mouse dorsal root ganglion cell culture
1:5000, overnight at 4°C
TBST (0.1%) containing 5% blotting-grade blocker
1:2000, 1 hr at room temperature
N/A
KwikQuantTM Imager
The band of β-actin (bottom) looks good, even the unspecific band does not cause trouble.
The antibody is ok
There is a nonspecific band
This β-actin antibody works well.