Great Human PDGFAA ELISA Kit from FisherSci

July 24, 2019

BlueskyReddit
UCLA
Medicine/CEIN
Research Scientist

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Company:

Thermo Fisher Scientific

Product Name:

Human Platelet Derived Growth Factor AA (PDGFAA) ELISA Kit, 96 tests

Catalog Number:

EKU06683

Since this is not a very popular product, I have been searching around for a long time and finally got this product for my experiments. I needed this ELISA kit to detect the PDGF-AA level in cell culture supernatants after exposed to our nanomaterials. This is a very good product. It's very sensitive and easy to process. The protocol is very clear and easy to follow. The results are very reliable. I have used several kits of this specific growth factor.

Experimental Design and Results Summary

Application

To detect PDGF-AA level in cell culture supernatants

Starting Material

Cell supernatants after exposed to nanomaterials

Tips

Co-culture of macrophage and epithelial cells

Results Summary

We used coculture of THP-1 with BEAS-2B cells to assess TGF-β1 and PDGF-AA production as relevant pro-fibrogenic growth factors, which in concert with IL-1β determines fibroblast proliferation and collagen deposition in the lung. Following incubation with 60 μg/mL f-MWCNTs for 24 h, IL-1β and growth factor production in the cellular supernatants were examined by ELISA. AP-MWCNTs served as a positive control. All MWCNTs, except for the COOH tubes, induced significant increased levels of IL-1β, TGF-β1, and PDGF-AA compared to untreated cells.

DOI or PMID #

N/A

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Summary

The Good

It's very sensitive and easy to process. The protocol is very clear and easy to follow. The results are very reliable.

The Bad

It's pre-coated. We cannot coat our own plate freely.

The Bottom Line

Although it is pre-coated, which gives us no opportunity to coat our own plates freely, it is sensitive and easy to process. The protocol is clear and easy to follow. The results are very reliable.

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