Very Good CST Antibody for Myosin IIA

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rabbit anti-myosin IIA

Catalog Number:

3403

Browse Similar Products: Myosin IIA Western Blot Antibodies

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I want to detect the expression of myosin IIA in retina by western.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse retina

Primary Incubation

1:1000, overnight at 4°C

Blocking Agent

TBST (0.1%) containing 506 5% blotting-grade blocker

Secondary Incubation

1:2000, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

KwikQuantTM Imager

Results Summary

The band is clear and clean.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

The antibody is specific and strong

The Bad

N/A

The Bottom Line

I definitely recommend this antibody for western blot.

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