BD Biosciences
Alexa Fluor® 700 Mouse Anti-Human IFN-γ
557995
B27
Browse Similar Products: IFN-Gamma Flow Cytometry Antibodies
Can detect IFNg production using as little as 0.25 uL/test in thawed frozen PBMCs stimulated with PMA and Ionomycin for 5 hours with BFA. Not particularly bright but clear separation.
Flow Cytometry
Frozen human PBMCs
Surface stain 30 min room temp (for CD markers)
PBS with 1% FCS
60 min on ice
N/A
Titrated 4 uL down to 0.125 uL per test. Clear separation without too much shift at 0.25 uL.
Used eBioscience fix/perm buffer
Can use small volumes
Not particularly bright
Recommend- have used with consistent results for years