Invitrogen
Isolectin GS-IB4, Alexa Fluor 488 conjugate
I21411
N/A
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My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I needed to do immunofluorescent staining on Dorsal root ganglion cell culture.
Immunofluorescence
Mouse dorsal root ganglion cell culture
1:200, overnight at 4°C
PBS containing 10% goat serum
CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software
IB4 just binds to the cell membrane, so you should stain IB4 individually in no-Triton incubation buffer before using any other antibodies for staining cytoplasm molecules. However, it is hard to adjust the concentration of this antibody. The signal is weak when low concentration is applied or non-specific when in high concentration. Sometimes you can get good picture but the result is not consistently good.
The antibody is ok
Not very specific
You should do some pre-experiments for getting a proper concentration.