Very Good CST Antibody for pGSK3β

Orthopedic surgery
Johns Hopkins University School of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rabbit anti-pGSK3β Ser9

Catalog Number:

5558

My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I needed to do immunofluorescent staining on retina cryostat sections.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse retina cryostat sections

Primary Incubation

1:200, overnight at 4°C

Blocking Agent

PBST (0.3%) containing 10% goat serum

Secondary Incubation

1:500, 1 hr at room temperature

Tertiary Incubation

N/A

Detection

CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software

Results Summary

The immunofluorescence signal of pGSK3β is quite specific and strong. This antibody works well.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody is specific and strong

The Bad

N/A

The Bottom Line

This antibody works well.

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