MilliporeSigma
Anti-CLIC5 antibody
AV35262
Browse Similar Products: CLIC5 Western Blot Antibodies
Chloride intracellular channel (CLIC) proteins belong to a family of highly conserved transport proteins found as both soluble and membrane-bound forms. In our lab we used this antibody for Western blots. I found this antibody worked well and gives very clear and crisp band of 46 kDa. This antibody can be used for wide variety of samples such as human, rat, mouse, rabbit, and horse. I have used for human and mice origin samples.
Western Blot
Mouse Heart
Antibody dilution (concentration) 1.25 μg/mL, Overnight at 4 degree
Odyssey blocking buffer (1:1) ratio with 1X PBS buffer
Goat anti-Rabbit IgG, LICOR (1:5000 dilution) at RT for 1hr
N/A
Scanned on Odyssey® CLx (auto scan 700 & s800).
I observed a good results with distinct band intensity between control vs experimental group. This worked very well for our experiment.
This is good antibody.
Optimization can vary with users and experimental samples
This is a good antibody for Western blot. I would recommend for WB.