BioLegend CD62L (Flow Cytometry) Stains Well in Th-Differentiation Experiments

June 21, 2019

BlueskyReddit
Laboratory Medicine and Pathobiochemistry - Molecular Diagnostics
Philipps-University Marburg, Center for Tumor- and Immunobiology (ZTI)
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-mouse CD62L Antibody

Catalog Number:

104405

Clone Number:

MEL-14

Isolated splenocytes from Balb/c mice were sorted for CD4+/CD62L+ and cultivated in-vitro for 5 days with and without Th-2 differentiation mix.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Ex-vivo Balb/c splenocytes

Primary Incubation

1:200 for 30min at 4°C

Blocking Agent

Fc-block (anti-CD16/32)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACS Canto II, analyzed in FlowJo

Results Summary

Positive cells were nicely stained. Background was minimal and easily accounted for with an isotype control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Easy to use, also in reasonable dilution

The Bad

None so far

The Bottom Line

The antibody does what it is created for. The CD62L was nicely detectable and the amount required for staining was reasonable in a 1:200 dilution. Background was minimal.

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