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CD73 (Ecto-5'-nucleotidase) Antibody
344016
AD2
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I am working with a multiplex immunofluorescence technique in which you can stain one single tissue section with more than 100 antibodies. I have a portfolio of more than 2000 different antibodies and have generated over 100,000 immunofluorescence images. I supervise several projects in which we stain different tissue samples, e.g., skin (melanoma, psoriasis), tumor, spleen, etc. For this experiment, our lab was interested in the characterization of immune cell subtypes in inflamed skin diseases like pyoderma gangrenosum and ulcus cruris. CD73 was one of the marker of our interest.
Immunofluorescence
Human skin
1:20; RT; 60 min
5% FBS; RT, 30 min
None
Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)
This antibody gives specific and moderate signals on frozen-tissue sections.
N/A
This antibody gives specific signal on frozen-tissue sections ....
....but with background
Overall an average/good antibody for IF