Perfect CD81 Antibody from BioLegend

Dermatology
University Hospital Erlangen
Core Facility Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD81 (TAPA-1) Antibody

Catalog Number:

349504

Clone Number:

5A6

I am working with a multiplex immunofluorescence technique in which you can stain one single tissue section with more than 100 antibodies. I have a portfolio of more than 2000 different antibodies and have generated over 100,000 immunofluorescence images. I supervise several projects in which we stain different tissue samples, e.g., skin (melanoma, psoriasis), tumor, spleen, etc. In this project, the pathogenesis of melanoma was investigated. With the MELC technology, we analyzed protein expression profiles in nevi and melanomas. Here we focused on the CD81expression.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human skin (melanoma)

Primary Incubation

1:100; RT; 60 min

Blocking Agent

5% FBS; RT, 30 min

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence signals (FITC) were detected by an inverted fluorescence microscope (Leica DM IRE2)

Results Summary

This antibody gives very specific and strong signals on frozen-tissue sections with no background. Publishable results!

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very specific signals, no background, easy to use!

The Bad

None

The Bottom Line

Great antibody for IF (not only FC)

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