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Notch 4 Antibody
349004
MHN4-2
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I am working with a multiplex immunofluorescence technique in which you can stain one single tissue section with more than 100 antibodies. I have a portfolio of more than 2000 different antibodies and have generated over 100,000 immunofluorescence images. I supervise several projects in which we stain different tissue samples, e.g., skin (melanoma, psoriasis), tumor, spleen, etc. For this project, our lab was interested in the investigation of the activation of ADAM proteases and their substrates (Notch 3 and 4) in inflammatory skin diseases like psoriasis.
Immunofluorescence
Human skin
1:10; RT; 60 min
5% FBS; RT, 30 min
None
Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)
This antibody gives specific but weak signals with some background on frozen-tissue sections.
N/A
Shows specific signals
Background staining
This antibody is ok, but not the best for IF