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CD45.2 Antibody
109808
104
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I am working with a multiplex immunofluorescence technique in which you can stain one single tissue section with more than 100 antibodies. I have a portfolio of more than 2000 different antibodies and have generated over 100,000 immunofluorescence images. I supervise several projects in which we stain different tissue samples, e.g., skin (melanoma, psoriasis), tumor, spleen, etc. In this project, the different phenotypes of immune cells in murine skin and lymph node were investigated. The goal was to distinguish donor (CD45.2) from host cells after adoptive transfer.
Immunofluorescence
Murine lymph node
1:50; RT; 60 min
5% FBS; RT, 30 min
None
Fluorescence signals (PE) were detected by an inverted fluorescence microscope (Leica DM IRE2)
This antibody gives very specific and strong signals on frozen-tissue sections with little background. Publishable results!
N/A
Specific signals, no background, easy to use!
Will buy and use it again. Highly recommended!