Confusing Protocol and Inconsistent Results

Johns Hopkins School of Medicine
Oncology
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Glucose-6-Phosphate Dehydrogenase (G6PD) Activity Assay Kit

Catalog Number:

12581

We were interested in looking at the G6PD activity in our murine T cells and tumor cells after we add our glutamine antagonism drug. However, the result of the assay was inconsistent and the protocol was confusing.

Experimental Design and Results Summary

Application

Activity Assay

Starting Material

Murine T cells

Tips

It is necessary to quantify proteins to standardize the loading sample amount.

Results Summary

The lysis buffer they provided was not compatible to our bradford protein assay. However the protocol never suggested any ways to quantify our protein amount, nor did it provide a standard we could use to generate a standard curve from.

DOI or PMID #

N/A

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Summary

The Good

All the materials were there, and making up the solutions was simple

The Bad

Lack of detail on the assay itself and the results were often inconsistent. In addition, the price of the kit was extremely high.

The Bottom Line

After looking through other companies for this same assay, it might be worth it to try other brands due to a better protocol and a lysis buffer that would not counteract with the bradford.

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