Thermo Fisher Scientific
Rabbit anti-myosin IIB
PA5-17026
N/A
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My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I want to detect the expression of myosin IIB in retina ganglion cells.
Immunofluorescence
Mouse retina cryostat sections
1:100, overnight at 4°C
PBST (0.3%) containing 10% goat serum
1:500, 1 hr at room temperature
CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software
I compared the signal of myosin IIB (green) in control group and in myosin IIB knock-out group. Under the same exposure time, the signal of myosin IIB in myosin IIB knock-out group is rather weaker than that in control group. However, there is still lots of unspecific binding. Moreover, this antibody can not be used to detect myosin IIB by western blot.
Myosin IIB can be detected by immunofluorescence staning
Non-specific
If you can find an antibody that is more specific, I wouldn't recommend this one.