Cell Signaling Technology
Rabbit anti-Cre recombinase
15036
Browse Similar Products: Cre Recombinase Immunofluorescence Antibodies
My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I wanted to check if the transduction rate of the pre-injected Cre recombinase virus was high enough.
Immunofluorescence
Mouse retina cryostat sections
1:500, overnight at 4°C
PBST (0.3%) containing 10% goat serum
1:500, 1 hr at room temperature
N/A
CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software
The signal of cre (red channel) clearly localizes with the signal of retina ganglion cells (green channel). The signal is strong and easily identified. With these results, I am sure that most of the target cells are infected by the cre virus.
The antibody is specific and strong
I would definitely use this antibody to detect cre virus transduction rate.