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Mouse anti-tubulin β3
801202
TUJ1
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My research focuses on molecular mechanisms of axon growth and guidance during neural development and axon regeneration after injuries to the central nervous system or peripheral nerves. For this experiment, I needed to do immunofluorescent staining on retina cryostat sections.
Immunofluorescence
Mouse retina cryostat sections
1:500, overnight at 4°C
PBST (0.3%) containing 10% goat serum
1:500, 1 hr at room temperature
N/A
CCD camera connected to a Zeiss inverted fluorescence microscope controlled by AxioVision software
The immunofluorescence signal of the Retina ganglion cells is quite specific and strong. This antibody works well to mark neurons.
Specific
I recommend that this antibody to be commonly used in immunofluorescent staining of neurons, no matter in CNS or in PNS.