Good CD271 Antibody from BD Biosciences

Genetics and Genomic Sciences
Mount Sinai
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE Mouse Anti-Human CD271

Catalog Number:

557196

Clone Number:

C40-1457

Browse Similar Products: CD271 Flow Cytometry Antibodies

I specialize in functional genomics and applying gene editing technologies to address immunology questions. I have recently developed a new barcoding system, which represents the largest known to date set of protein-based reporters (doi.org/10.1016/j.cell.2018.09.022). This product is an excellent antibody for detection of dNGFR transduced cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human HEK-293T cells transduced with NGFR expressing lentiviral vector

Primary Incubation

1:250, 30 min on ice

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD Fortessa

Results Summary

High specific signal, minimal background; excellent antibody to detect cells transduced with NGFR expressing lentiviral vector.

DOI or PMID #

https://doi.org/10.1016/j.cell.2018.09.022

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use; low background

The Bad

N/A

The Bottom Line

Excellent antibody, much better than ME20.4 clone which gives higher background and much lower specific signal.

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