Good Human CD127 Antibody from BioLegend

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-human CD127 (IL-7Rα) Antibody

Catalog Number:

351331

Clone Number:

A019D5

Our lab studies T cells across different organs in the human bodies and how they develop and function. One of our targets of interest in CD127 so we chose this bright antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human lung cells

Primary Incubation

Cells were stained in 100 ul FACS buffer with antibody at 1:100; for 1 hour on ice and in the dark. Cells were washed with FACS buffer and run in 100 uL FACS buffer.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

This antibody allowed us to clearly see and analyze CD127 expression on human T cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This is a good antibody for human cell CD127 expression.

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