Great TNF Alpha Antibody for Mouse from BioLegend

M&I
COLUMBIA UNIVERSITY
Lab Director / Manager

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Quality of Results

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Company:

BioLegend

Product Name:

Anti-mouse TNF-a Alexa Fluor 647

Catalog Number:

506314

Clone Number:

XMG1.2

One of the focuses in our lab is to study the function of T cells during an influenza infection in a mouse model. We needed a reliable TNF alpha antibody to study the upregulation of this cytokine.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lung cells

Primary Incubation

Cells were fixed in Tonbo fix/perm buffer and then stained at 1:50 in perm buffer for 30 minutes at room temperature.

Blocking Agent

FACS

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSR II

Results Summary

We were able to clearly see the positive and negative peaks of TNF alpha expression in our mouse cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright and stable

The Bad

None

The Bottom Line

This antibody is good for staining intracellular TNF alpha in mouse cells.

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