Miltenyi Anti-RORgt-APC, Human and Mouse, that Works

June 05, 2019

BlueskyReddit
Innate Immunity
Deutsches Rheuma-Forschungszentrum
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

Anti-RORγ (t)-APC, human and mouse

Catalog Number:

130-103-838

Clone Number:

9E2

Retinoid-related orphan receptor-γ (RORγt) is a transcription factor which is expressed almost exclusively in lymphocytes, both innate and adaptive. It is essential for thymopoiesis and the development of several secondary lymphoid tissues, including lymph nodes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Cultured cells

Primary Incubation

15 min, 37°c

Blocking Agent

0.5% BSA in PBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

FACSymphony, software FACSDiva

Results Summary

Bright and strong resolution in cultured cells, however, in fresh tissues staining is problematic and must be done immediately.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Can be bright and good resolution in cultured cells

The Bad

Staining not stable on fresh human tissues

The Bottom Line

Would recommend, but requires high optimization for human samples

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