BioLegend
APC/Cyanine7 anti-human CD14 Antibody
301820
M5E2
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining monocytes (CD14+) in peripheral blood of a healthy donor (HD).
Flow Cytometry
Whole blood of a healthy donor
Anti-CD14 APC-Cy7, 4 C, 15 min
Fc block
N/A
Flow cytometry
Monocytes are positive for CD14, while B lymphocytes do not express CD14.
It works well.
There is a bit of background staining.
It is a good option to stain CD14.