Easy Way to Dialyze from Thermo

May 24, 2019

Centre for Cellular and Molecular Biology
Laboratory of t-RNA Transcription
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

Slide-A-Lyzer Dialysis Cassette (Extra Strength) 3-12 ml capacity

Catalog Number:

66810

The aim of our experiment is to dialyze the protein samples against the dialyzing buffer. The protein sample was loaded in the dialysis cassette using the provided syringe with the kit. After overnight dialysis, samples were taken out from dialysis cassette and analyzed.

Experimental Design and Results Summary

Application

Protein purification

Starting Material

Antibody in elution buffer

Tips

Hydrate cassette in dialyzing buffer for 2 minutes.

Results Summary

Protein was purified and concentration was measured by using 96-well plate reader.

DOI or PMID #

N/A

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Summary

The Good

Easy to use, no leakages, and easy to fill and take out sample from dialysis cassette.

The Bad

N/A

The Bottom Line

We have ordered 3-12 ml cassette and it can easily accommodate 15 ml of sample. Just 2 min hydration and it is ready to use. Simple to fill the sample in the cassette with the provided syringe and easy to take out the sample. No leakage of sample and complete recovery of the sample.

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