Agilent Technologies
QuikChange Lightning Site-Directed Mutagenesis Kit
210518
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My main goal is to create mutations in DNA sequence finally to alter the amino acid sequence of the protein. I used this kit many times to create a single mutation in the DNA sequence, and it worked perfectly almost every time with high efficiency of mutation. I also used the same kit to create multiple site-directed mutations (single primer with multiple mutations) and it worked fine.
To create mutations in the DNA seq/to change the amino acid sequence
Plasmid DNA
I used only 1 micro liter of DpnI enzyme instead of two and increased incubation time - works perfectly.
I did almost 20 reactions to create different single and multiple mutations in plasmid DNA (plamsid sizes: 6-12kb) and all of them worked perfectly with no extra unwanted mutations. Heat shock method of transformation with other E.coli strains other than the provided XL-gold strains also worked perfectly for this kit.
N/A
Quick and high efficiency rate for mutation without errors
Price - Too much for 10 reactions
Quick and easy to use to create perfect single and multiple mutations in DNA sequence. Highly recommended.