Good Antibody for Human CTLA4 Staining - Thermo

May 30, 2019

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD152 (CTLA-4) Monoclonal Antibody (14D3), PE, eBioscience™

Catalog Number:

12-1529-42

Clone Number:

I used this antibody to stain CTLA4 expression in CD4+Foxp3+ and CD4+Foxp3- T-cells in human thymus.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymocytes

Primary Incubation

1:100 for 3h at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct expression pattern of CTLA4 between populations analyzed. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

I stained the cells after fixation and permeabilization to measure both intracellular and surface CTLA4 expression.

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Nothing noted

The Bottom Line

It's worth buying.

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