Excellent Antibody for Human CD45RA Staining - Thermo

May 29, 2019

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD45RA Monoclonal Antibody (HI100), APC, eBioscience™

Catalog Number:

17-0458-42

Clone Number:

I used this antibody to distinguish CD45RA-Foxp3+ and CD45RA+Foxp3- T cells in human. This antibody stained well and showed distinct populations when used in combination with FOXP3 antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human thymocytes

Primary Incubation

1:100 for 3h at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

It's worth buying

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