Good Antibody for Mouse CXCR5 Staining - BioLegend

May 14, 2019

Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse CD185 (CXCR5) Antibody

Catalog Number:

145511

Clone Number:

I used this antibody to stain CXCR5hiPD1hi follicular helper T-cells in mouse. This antibody stained well and showed distinguished CXCR5hiPD1hi population when used in combination with PD1 antibody.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

These results were obtained post immunization of mice which is needed for T follicular cell diffrentiation.

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Somewhat weak staining

The Bottom Line

Its worth buying.

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