Excellent Antibody for Mouse FR4 Staining - Thermo

May 16, 2019

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

FR4 Monoclonal Antibody (eBio12A5), PE-Cyanine7, eBioscience™

Catalog Number:

25-5445-82

Clone Number:

I used this antibody to stain CD73hiFR4hi anergic T-cells in mouse. This antibody stained well and showed distinguished FR4+ population between naïve and anergic T-cells when used in combination with CD44 and CD73 antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Ease of optimization and reproducible results

The Bad

Expensive

The Bottom Line

It's worth buying this antibody.

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