Good Antibody for Mouse CD73 Staining - Thermo

May 10, 2019

BlueskyReddit
Microbiology and Immunology
UIC
Postdoctoral Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), eFluor 450, eBioscience™

Catalog Number:

48-0731-82

Clone Number:

I used this antibody to stain CD73hiFR4hi anergic T-cells in mouse. This antibody stained well and showed distinguished CD73+ population between naïve and anergic T-cells when used in combination with CD44 and FR4 antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:200 for 3h at 4 degree

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

This antibody stained well and showed distinct population. I am able to get reproducible results.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good specificity and ease of optimization

The Bad

Expensive

The Bottom Line

It's worth buying this antibody

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