Good LC3 Stainings

May 10, 2019

BlueskyReddit
Neuropathology
Charité-Universitätsmedizin Berlin
Graduate Student

Overall

Quality of Results

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Company:

Sigma

Product Name:

Anti-LC3B antibody

Catalog Number:

L7543

Clone Number:

The LC3 antibody offered by Sigma nicely stains LC3 positive autophagosomes. The signal is precise and suitable for super resolution microscopy.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Neonatal microglia cells from mice

Primary Incubation

1:200 at RT for 1h

Blocking Agent

2% BSA

Secondary Incubation

Alexa568-conjugated secondary antibody (Invitrogen, 11011) for 1 h (1:500) at RT

Tertiary Incubation

N/A

Detection

Fluorescence, Confocal and super resolution microscopy

Results Summary

Methanol fixation is necessary for LC3 staning of cultured cells. If the protocol is followed the LC3 antibody works very nicely.

DOI or PMID #

30617086

Additional Notes

N/A

Related Categories

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Summary

The Good

Nice and clear detection of LC3-positive autophagosomes

The Bad

Requires specific fixation protocol

The Bottom Line

Good antibody for staining of LC3 positive autophagosomes

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