Excellent Caspase-3 Antibody from Boster Bio

May 07, 2019

BlueskyReddit
Neurosurgery
Duke University Hospital
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Boster Biological

Product Name:

Anti-Caspase-3(P10) primary antibody

Catalog Number:

PA1302-1

Clone Number:

I purchased this product to stain rat spinal cords exposed to injury. The injured spinal cords showed apoptotic cells obviously.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Rat spinal cord

Primary Incubation

Overnight at 4C (1:200 dilution)

Blocking Agent

5% goat serum

Secondary Incubation

1 hour at room temperature (1:500 dilution)

Tertiary Incubation

Dapi mounting solution

Detection

Fluorescent microscope

Results Summary

Injured spinal cord sample showed high apoptosis compared to control (uninjured) spinal cord.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Ease of use, reliable

The Bad

None

The Bottom Line

Excellent product and easy to use.

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