Good Anti-Human CD3 Pacific Blue from BD Biosciences

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Pacific Blue Mouse Anti-Human CD3

Catalog Number:

558117

Clone Number:

Browse Similar Products: CD3 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) in health and disease. Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining CD4 T cells (CD3+CD4+) in whole blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human whole blood

Primary Incubation

1/50, 20min, 4C, anti-CD3

Blocking Agent

Fc block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometer

Results Summary

We could stain CD4+CD3+ T cells in peripheral blood.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

It works well

The Bad

It is not very bright

The Bottom Line

It is a good option to stain human CD3.

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